Protocols
This is how we do it
Step-by-step guides detailing some of our frequently used methods
Stem cell-derived NGN2-accelerated Progenitors (SNaPs)
Conventional approaches for generating neural progenitor cells (NPCs) from human stem cell starting materials require multiple weeks for induction. Here, we describe our two-day induction protocol for creating high purity cultures of NPCs that has been successful in over 100 different cell lines.
Three-dimensional Cerebral Organoids & Neurospheres
There are several established protocols for creating 3D human cerebral organoid and neurosphere models of disease. Here, we describe our protocols for SNaP-derived neurosphere and "quick and dirty" organoid production. These are well-adapted for multi-line investigations of the early developing brain.
Immunostaining of in vitro neural cells and organoids
Our ability to measure the intensity and localization of proteins in a cell or tissue is key to understanding the biology of brain development. Here, we describe our techniques for immunostaining both 2D and 3D in vitro cultures that can then be imaged through high-resolution confocal microscopes or plate readers.